Research Engineer position to investigate early breast tumorigenesis using lineage tracing, organoid
岗位职责
Investigating the early mechanisms of breast tumorigenesis This project aims to investigate the early events driving breast tumor initiation and progression.
The research will rely on a lineage-tracing model targeting luminal epithelial cells to track the earliest stages of tumor transformation.
The project will combine 3D cell culture models (organoids) and mouse xenograft models.
Genetic manipulation of epithelial cells will also be performed, including functional screening approaches based on
CRISPR technology.
The characterization of these models will involve confocal microscopy imaging and spectral flow cytometry analyses.
The successful candidate will join the Epithelial Stem Cells and Cancer team, co-led by Emmanuelle Charafe-Jauffret and Christophe Ginestier, at the Marseille Cancer Research Centre (CRCM),
INSERM U1068, Marseille, France.
申请条件
- 具备乳腺癌肿瘤发生早期机制研究背景
- 具有谱系追踪模型相关研究经验
- 熟悉3D细胞培养模型（类器官）技术
- 具有小鼠异种移植模型操作经验
- 掌握上皮细胞基因操作技术
- 具备CRISPR功能筛选技术经验
- 熟悉共聚焦显微镜成像分析
- 具备光谱流式细胞术分析经验
- 具有肿瘤生物学或相关领域博士学位
- 具备独立开展科研工作的能力
- 具有良好的团队协作和沟通能力
- 具备良好的英语读写和交流能力
雇主简介
INSERM U1068 is a research unit focused on cancer research, specifically investigating the early mechanisms of breast tumorigenesis using lineage-tracing models, organoids, and mouse xenografts.